How to Read an Ovulation Test

Written by:, PhD, Founder and Inventor of the Proov test — the first and only FDA-cleared test to confirm successful ovulation at home.

Written by: Dr. Amy Beckley, PhD, Founder and Inventor of the Proov test the first and only FDA-cleared test to confirm successful ovulation at home.

Written on 10/17/20

Updated on 7/18/22

how to read an ovulation test

Using ovulation tests can help you better time intercourse around ovulation and get pregnant faster!

Ovulation tests (also known as ovulation predictor kits or OPKs) are great tools to help you on your trying-to-conceive journey. They’re simple at-home urine tests that measure luteinizing hormone (LH) to help you identify the timeframe when you’re most likely to ovulate — so you know the best days to try. The result: a better shot at getting pregnant faster!

How do you read an ovulation test? 

Ovulation test technology is similar to at-home pregnancy tests: you dip a test strip in urine, wait a while, and watch for your results to show up as lines on the test results window on the strip. (Always follow your test’s instructions carefully, testing at the right time of day as directed and allowing your test strip to develop for the specified number of minutes.)

Similar to an at-home pregnancy test, a positive ovulation test will show two lines. The first line, often pink, is the “control line.” This indicates the test was used correctly and is working.

The second line or “test line” represents the positive result. If this line is as dark or darker than the control line, then your test is positive. That means ovulation is expected in the next 12-36 hours, and now is a great time to try for a baby.

Do I need a special device to read an ovulation test?

Some brands do market fertility hormone tests that require costly extra devices, but they’re usually not necessary.  Most standard ovulation tests can be read by eye.

Top-quality OPKs like Proov Predict also work with an app on your phone for goof-proof scanning and results — and that’s what we recommend. Although Proov Predict test strips can also be read by eye, the free Proov Insight app gives reliable readings and tracks your cycle data for you. Plus you get additional insights and tips to help you get pregnant fast.

Reading your test with an app is great because it takes away any guesswork. Do be careful, though, to scan your test on a plain flat surface in good lighting with no harsh shadows falling across the results window. This will ensure you get the most accurate results.

how to read an ovulation test

Reading your Proov Predict test with the free Proov Insight app is great because it takes away any guesswork.

Does a result with two lines always mean an ovulation test is positive?

No, it does not. It’s normal for urine to contain a low level of luteinizing hormone (LH) every day. This means it’s likely that your ovulation test will always show two lines. But when LH is low, the test line will be faint, lighter than the control line.

As ovulation approaches, the amount of LH in your urine increases, which means the test line will become darker. Once it’s as dark or darker than the control line, your test is positive and you can rest assured that ovulation is expected in the next 12 to 36 hours.

As soon as you receive your positive result, you’re in your predicted fertile window. Time to get busy!

In fertility hormone testing, are positive results always displayed as two lines?

No, it’s a bit more complicated than that. Since different hormones are measured in different ways, tests don’t all work the same. For instance, when measuring PdG with Proov Confirm — a key step in confirming that successful ovulation actually occurred – the presence of two lines is actually a negative result.

This can get a little confusing, which is why we recommend the Proov Insight app, which works with all Proov hormone tests. Our app makes tracking hormones easy with daily prompts, and scans and interprets your results for you.

What should I do if my ovulation test is negative?

If your ovulation test is negative, it likely means your body just needs a bit more time to get ready to release an egg. Keep testing as directed until you observe a positive result. If you never get a positive ovulation test, we recommend consulting your doctor.

Once you get a positive ovulation test, you no longer need to continue testing LH for that cycle. However, we highly recommend testing PdG with Proov Confirm to confirm that successful ovulation actually occurred. It’s a piece of the fertility puzzle that is often overlooked, and can make all the difference.

My ovulation test was positive — now what?

If you’re trying to conceive, this is great information to have! A positive ovulation test means you’re in your fertile window, and ovulation is expected in the next 12 - 36 hours. Since sperm remain viable inside a woman’s body for several days after intercourse or insemination, right now is a great time to try for a baby.

While it is important to time intercourse during your fertile window, try not to stress about trying multiple times or nailing to-the-minute precision timing. As long as you have intercourse a few days leading up to ovulation, you’re still giving yourself a good chance at conception. So do what’s right for you.

Finally, don’t forget to follow up in the second part of your cycle by testing PdG with Proov Confirm — the first and only FDA-cleared at-home test that confirms successful ovulation actually occurred.

how to read an ovulation test

Are all ovulation tests the same?

Yes and no. All ovulation tests or OPKs basically do the same thing: they measure LH in urine. But with more than 50 such options on the market, you’ve got plenty of choices. If studying up on all the brands seems overwhelming, don’t worry: I tried more than a dozen and shares everything she learned in this article. (Yes, we’re really into testing at Proov!)

Proov Predict & Confirm kits are an especially convenient way to test because they include easy-to-use LH tests and PdG tests in one product. That means you can predict timing for ovulation AND follow up later in your cycle to confirm that successful ovulation actually occurred.

This two-step method not only helps you know the best time to try, but also helps you rule out any unexpected issues with ovulation and get set up for success. Since studies show that ovulatory disorders are the number-one cause of infertility, predicting and confirming successful ovulation is key.

For people who love getting as much information as possible, we also recommend Proov Complete — our most advanced fertility testing system. While typical ovulation tests measure LH to identify a 1-2 day fertile window, Proov Complete looks at all four major cycle hormones to identify an even longer fertile window of up to 6 days, and confirms successful ovulation by measuring PdG.

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