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Frequently Asked Questions

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We’ve provided answers to common questions below, but if something still isn’t clear, please reach out —

Proov tests work by detecting the amount of the hormone PdG in urine over multiple days to confirm successful ovulation.

PdG is the urine metabolite of progesterone – the hormone released from the ovary after ovulation. Sustained elevated levels of PdG can confirm that ovulation has successfully occurred.

Progesterone is the precursor of urinary pregnanediol glucuronide (PdG). Progesterone gets secreted in the blood, acts on the uterus to prepare it for pregnancy, passed through the liver, gets metabolized to PdG and secreted into the urine.

After ovulation the ovary releases progesterone into the bloodstream and helps prepare the uterus for embryo implantation. Progesterone gets metabolized by the liver and secreted into the urine as PdG. Published studies show Progesterone in serum directly correlates with urine PdG after ovulation.

Testing progesterone in serum via a one-time blood draw is plagued by inconsistency and thus lacks good diagnostic power. Studies show that since progesterone in released from the ovaries in pulses, during a single 24-hour period, serum progesterone fluctuations and has a huge range, from 3 to over 20 ng/ml.

However, studies on PdG (a urine metabolite of progesterone) show that measuring PdG daily in urine after ovulation produced more consistent results and was a better overall way to assess the luteal phase function (proper ovulation) of the menstrual cycle. By measuring PdG in daily urine on four consecutive days, Proov allows women to get a more complete picture of whether or not they have successfully ovulated.

PdG levels must be at a certain level to ensure proper ovulation. Inadequate PdG production can lead to problems with ovulation, making it difficult to conceive.

Ovulation predictor kits typically measure a hormone called luteinizing hormone (LH), which is released 24-48 hours before ovulation occurs. Proov tests measure PdG, a metabolite of progesterone, which is produced after you ovulate. Therefore, OPKs help to predict when ovulation will occur and Proov tests allow women to confirm that ovulation has successfully occurred.

Each test is individually packaged in a foil pouch and each kit contains 5, 9, or 18 test strips, depending on the pack purchased. Each test will have a shelf life of 15 months from the date of manufacture. We guarantee at least 6 months of shelf life for each purchase from our website. Please contact us for specific lot information.

A laboratory study has shown Proov tests to be over 99% accurate in detecting Pregnanediol Glucuronide (PdG) in urine.

Yes, Proov tests are FDA Cleared under 510(k) number K191462. Proov tests are manufactured in an FDA registered facility under cGMP conditions. Each batch of tests meet all required specifications before being sent to consumers.

No. Proov tests are designed to give either a positive or negative result and are therefore, not quantitative. Proov turns positive when PdG levels are 5 ug/ml of above. In a recent WHO funded research study, proven fertile women were monitored daily for PdG (the urine metabolite of progesterone) and E1G (the urine metabolite of estrogen) to determine healthy levels of PdG and E1G. The results showed the PdG was over 5 ug/ml 7 through 10 days after ovulation in healthy, fertile women. Therefore, research suggests that a healthy ovulatory cycle creates PdG levels of 5 ug/ml from 7 to 10 days after ovulation.

Sometimes. While PdG is a urine metabolite of progesterone, the two measurements don't always agree. This is due to high fluctuations in serum progesterone levels over the course of a single day. But studies show PdG levels are actually a good way to track the average level of progesterone you produce after ovulation.

Proov measures a hormone called PdG. PdG is a urine metabolite of progesterone. While both Progesterone and PdG are released after ovulation and can be used to confirm ovulation, the two are not the same.
1)Testing progesterone requires a blood draw or finger pick and long wait times to results. PdG is found in urine so testing only requires collection of first morning urine.
2) In published studies, PdG was found as accurate as serum progesterone measurements for confirming ovulation. In fact, some studies suggest PdG is better because it does not fluctuate as much as progesterone does in blood, allowing women to get a more complete picture of if they are ovulating properly.

Ovulation Predictor Kits (LH) and pregnancy tests (HCG) work by “sandwiching” the hormone between two antibodies. As such, a second line indicates a positive test for LH and HCG tests. Since the metabolite of progesterone is so small, this same technique does not work for Proov tests. Instead, we use a different method that indicates a positive test via 1 line instead of 2. Similar methods are used to measure estrogen and detect drugs in urine (eg. THC – Marijuana).

If you see 2 pink or purple lines, one in the test area and one in the control area, the test is considered negative.

Hormonal birth control and medications containing natural or synthetic progesterone will affect your results.

Proov tests do not replace medical advice. If you are not getting positive results, you should consult your physician and tell them about this information so they can properly diagnose you.

Absolutely! We are always here to help. There are several ways to reach us, email us at, on our private Facebook group ( or on Instagram @proovtest.

Proov tests on sold on our website, on Amazon, and in select Target stores.

Unfortunately, no. Proov tests are currently only available for shipping to the United States, Canada, and Australia. Please contact us if you are interested in discussing distribution opportunities in your country:

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Full instructions on how to use Proov can be downloaded here. Additionally, each purchase of Proov will include full instructions for use.


Proov was designed to help monitor ovulation to aid in conception. While progesterone remains high during pregnancy, Proov has not been clinically validated for use during pregnancy.