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Frequently Asked Questions

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We’ve provided answers to common questions below, but if something still isn’t clear, please reach out —

Proov tests measure the urine metabolite of the hormone progesterone called Pregnanediol Glucuronide, or PdG for short.

Progesterone is very low in the beginning of the menstrual cycle. Once ovulation takes place, the ovaries start producing high levels of progesterone. Additionally, progesterone levels will rise when a woman becomes pregnant and remain elevated throughout her pregnancy.

Progesterone is the hormone that is released after ovulation and helps prepare the uterus for conception. Progesterone also acts to maintain the uterine lining to support the pregnancy after the fertilized egg implants.

Progesterone levels must be at a certain level to ensure proper ovulation is taking place and the uterus is ready to receive the fertilized embryo. Inadequate progesterone production can lead to infertility, early pregnancy loss, premenstrual syndrome (PMS), brittle bones, weight gain (especially around the midsection), dry skin, low sex drive, and low energy.

Ovulation predictor kits typically measure a hormone called luteinizing hormone (LH), which is released 24-48 hours before ovulation occurs. Proov tests measure a metabolite of progesterone, which is produced after you ovulate. Therefore, OPKs help to predict when ovulation will occur and Proov tests allow women to confirm that ovulation has occurred.

Each test is individually packaged in a foil pouch and are sold in sets of 7. Each test will have a shelf life of >12 months from the date of manufacture.

In lab studies, Proov tests were 99% accurate for detecting Pregnanediol Glucuronide (PdG) in urine.

Yes, Proov tests are FDA compliant. The tests are manufactured in an FDA inspected and certified facility under cGMP conditions. Each batch of tests meet all required specifications before being sent to consumers.

No. Proov tests are designed to give either a positive or negative result and are therefore, not quantitative. The threshold for a positive test is 5 ug/ml. This threshold was selected because it indicates ovulation has occurred.

Ovulation Predictor Kits (LH) and HCG pregnancy tests work by sandwiching the hormone between two antibodies. As such, a second line indicates a positive test for LH and HCG tests. Since the metabolite of progesterone is so small, this same technique does not work for Proov tests. Instead, we use a different method that indicates a positive test via 1 line instead of 2. Similar methods are used to measure estrogen and detect drugs in urine.

If you see 2 pink or purple lines, one in the test area and one in the control area, the test is considered negative.

Alcohol does not affect your results. However, hormonal birth control and medications containing natural or synthetic progesterone will affect your results.

Proov tests do not replace medical advice. If you never get a positive result, you should consult your physician and inform them of your results so they can properly diagnose you.

Yes! We are happy to help. Please contact us at, through our website –, on Facebook (search Proov Users) or on Instagram @proovtest.

Proov tests can be purchased via our website or on Amazon.

Unfortunately, no. Proov tests are currently only available for shipping to the United States, Canada, and Australia. Please contact us if you are interested in discussing distribution opportunities in your country:

Contact us! We would love to hear from you. Please reach out at

Full instructions on how to use Proov can be downloaded here. Additionally, each purchase of Proov will include full instructions for use.


Proov was designed to help monitor ovulation to aid in conception. While progesterone remains high during pregnancy, Proov has not been clinically validated for use during pregnancy.