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Proov Complete 101

Written by:, PhD, Founder and Inventor of the Proov test — the first and only FDA-cleared test to confirm successful ovulation at home.

 Updated on 7/24/23

Welcome! If you’re here, you’ve likely already purchased Proov Complete and are gearing up for testing. We are so excited for you to get started and understand how to use Proov!

Complete provides the most comprehensive view of your hormone levels and personalized information to help you get pregnant. In order to get the most out of your test kit, we recommend following your specific Proov complete testing system protocol as dictated by the Proov Insight app.

While we cannot specifically speak to every testing scenario — as your specific protocol is determined by the cycle info you input into the app — we can provide a roadmap view of what you can expect. So, let’s dive in!


Keep reading for a roadmap of the Proov Complete testing protocol!

First things first…

Once you receive your Proov Complete kit and get set up in the Proov Insight app, you will be prompted to input some simple cycle data. The Proov Insight app uses this data to determine your personalized testing schedule.

Important note! Complete testing must begin on the 5th day after the start of your period. We call this cycle day 5 (cycle day 1 = the first day of your period). If you receive your kit after cycle day 5 or missing cycle day 5 testing, you will need to wait until the next cycle to start your testing protocol.

From there, it’s time to Proov!

Below is an overview of an example testing protocol, based on a 30 day cycle. For more specifics and details, continue scrolling.

Proov Complete 101

Follicular Window Testing

In the Proov Insight app, follicular window testing involves a few key steps:

  1. Establishing baseline hormone levels.
  2. Assessing ovarian reserve. 
  3. Identifying the fertile window.
Establishing baseline hormone levels

On cycle day 5, you will need to take a FSH (follicle stimulating hormone) test and a Multihormone test using first morning urine. These tests will help us identify your baseline hormone levels — i.e. your hormones’ “starting point” each cycle.

This is a critical step as it allows us to accurately identify and assess the chances in your levels across your cycle, by giving us something to compare them to later on.

Assessing ovarian reserve

Next, you’ll take 2 more FSH tests on cycle days 7 and 9 (so you’ll have 2 days in between each FSH test). Your FSH levels give insight into your ovarian reserve, or how many eggs you have left.

Since, like every hormone, FSH can fluctuate, it’s important to test on all 3 testing days.

Identifying the fertile window

The fertile window is the last piece of follicular window testing. Using your previously inputted cycle data, the Proov Insight app will prompt you exactly which day to begin testing for your fertile window.

You will take one Multihormone test each day — using only first morning urine — to monitor changes in E1G (estrogen marker), LH (luteinizing hormone), and PdG (progesterone marker. A rise in Proov E1G levels is the first sign that your fertile window is opening, meaning it’s time to get busy!

From there, you’ll continue using a Multihormone test daily. A surge in LH will identify your 2 most fertile days that cycle (meaning ovulation is hopefully coming soon!) and begins the “days past peak” count — which will become important in the second half of testing.

You’ll still continue testing with a Multihormone test each morning until the app confirms a rise in PdG. This not only confirms that ovulation did in fact occur, but also closes your fertile window, meaning any intercourse after this point is less likely to result in conception.

Now, onto the last piece of testing!

Luteal Window Testing

While a lot of testing happens in the first of your cycle, in our opinion the luteal window is where the real Proov magic happens!

Note: You must save 4 Multihormone tests for luteal window testing. If you are running low on tests before the luteal window or run out of tests, please contact us.

The app will prompt you to test on 4 key days during the luteal window known as the implantation window. During these 4 days, we’ll specifically be looking at PdG to develop your Ovulation Score — an assessment of your PdG levels after ovulation that suggest how conception-ready your body is.

To put it simply, higher PdG levels allow for a higher chance at conception and lower PdG levels may make it a little more difficult to successfully conceive.

While you will receive individual PdG values each day during this testing window, we recommend waiting until you have all 4 tests before making any conclusions about your levels. Like all hormones, PdG levels can fluctuate slightly from day to day, so it’s important to understand the overall pattern in your levels.

The Grand Reveal

Ta-da! After completing all 4 days of luteal window testing, you will receive your personalized Ovulation Score and Ovulation Insights. You’ll be able to see an overview of your hormones, an analysis of your score, and a personalized action plan to help you get pregnant.

If you have any questions before, during, or after testing, please don’t hesitate to reach out to us! Our dedicated customer support team is always here to help, every step of the way.

Happy Prooving!

Have questions? Email us!