Frequently Asked Questions

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We’ve provided answers to common questions below, but if something still isn’t clear, please reach out —

What does Proov measure?

Proov tests measure the urine metabolite of the hormone progesterone called Pregnanediol Glucuronide, or PdG for short.

When is progesterone produced?

Progesterone is very low in the beginning of the menstrual cycle.  Once ovulation takes place, the ovaries start producing high levels of progesterone.  Additionally, progesterone levels will remain elevated when a woman becomes pregnant and remain elevated throughout her pregnancy.

What exactly is Progesterone?

Progesterone is the hormone that is released after ovulation and helps prepare the uterus for conception. Progesterone also acts to maintain the uterine lining to support the pregnancy after the fertilized egg implants.

Why should I test my Progesterone levels?

Progesterone levels must be at a certain level to ensure proper ovulation is taking place and the uterus is ready to receive the fertilized embryo. Inadequate progesterone production can lead to infertility, early pregnancy loss, premenstrual syndrome (PMS), brittle bones, weight gain (especially around the midsection), dry skin, low sex drive, and low energy.

How are Proov tests different from ovulation predictor kits (OPKs)?

Ovulation predictor kits typically measure a hormone called luteinizing hormone (LH), which is released 24-48 hours before ovulation occurs.  Proov tests measure a metabolite of progesterone, which is produced after you ovulate.  Therefore, OPKs help to predict when ovulation will occur and Proov tests allow women to confirm that ovulation has occurred.

How are the tests packaged and when do they expire?

Each test is individually packaged in a foil pouch and are sold in sets of 7.  Each test will have a shelf life of >12 months from the date of manufacture.

How accurate are Proov Tests?

In lab studies, Proov tests were 99% accurate for detecting Pregnanediol Glucuronide (PdG) in urine.

Are Proov tests clinically validated?

In an IRB-approved clinical study we monitored 190 menstrual cycles. 87% of women reported positive Proov test results 2-9 days after peak fertility was observed and none reported seeing a positive Proov test prior to peak fertility. Studies have shown that up to 46% of women can have LH surges (positive OPK results), but still fail to ovulate. Therefore, Proov tests allow for more accurate ovulation testing than using OPKs (LH tests) alone.

Do Proov tests meet FDA requirements?

Yes, Proov tests are FDA compliant.  The tests are manufactured in an FDA inspected and certified facility under cGMP conditions.  Each batch of tests meet all required specifications before being sent to consumers.


Are Proov tests quantitative?

No.  Proov tests are designed to give either a positive or negative result and are therefore, not quantitative.  The threshold for a positive test is 5 ug/ml, which is considered the minimum threshold of PdG needed to support conception and pregnancy.

Why is a single line a positive result and two lines are a negative result?

Ovulation Predictor Kits (LH) and HCG pregnancy tests work by sandwiching the hormone between two antibodies.  As such, a second line indicates a positive test for LH and HCG tests.  Since the metabolite of progesterone is so small, this same technique does not work for Proov tests.  Instead, we use a different method that indicates a positive test via 1 line instead of 2.  Similar methods are used to measure estrogen and detect drugs in urine.

The test line is lighter than the control line. What does that mean?

If you see 2 pink or purple lines, one in the test area and one in the control area, the test is considered negative.

When do Proov tests typically turn positive?

Proov tests turn positive anywhere between 2-7 days after ovulation and remain positive until 1-3 days before the next menstrual period or if conception has occurred, will remain positive through the pregnancy.

I still see a very faint, greyish line in the test area. Is my test positive?

Yes, occasionally there are compounds in urine that react with the test line area and create a very slight greyish evaporation line. If you test has a pink or purple line in the control area and no pink color in the test line area, it is considered positive.  If you have any questions about reading your test, please contact us at

Do alcohol or medications affect the results?

Alcohol does not affect your results. However, hormonal birth control and medications containing natural or synthetic progesterone will affect your results.

I have taken these tests as directed and I never get a positive result. Do I have low progesterone?

Proov tests do not replace medical advice.  If you never get a positive result, you should consult your physician and inform them of your results so they can properly diagnose you.

Do Proov tests work to detect progesterone for everyone?

Proov tests work for most women.  However, our studies show that 7-12% of women do not metabolize progesterone into PdG at high enough levels to get a positive Proov test result.  While these women may show sufficient progesterone levels via a blood test, Proov tests will not turn positive.  If you suspect that you may be in this group of women, please contact us at

I am unsure of my test results. Can you help?

Yes!  We are happy to help.  Please contact us at, through our website –, on Facebook (search Proov Users) or on Instagram @proovtest.

How many Proov tests do I need per month?

There are several factors that affect the number of tests you will need per month.  These factors include cycle length, use of ovulation predictor kits (OPKs), basal body temperature charting, and cervical mucus tracking.  The average user will need 1-5 tests per month.  A woman needs a single positive test to confirm the presence of elevated progesterone metabolites in urine, and thus confirm ovulation.

Where are Proov tests sold?

Proov tests can be purchased via our website or on Amazon.

I live in Europe. Can I get a Proov test kit?

Unfortunately, no.  Proov tests are currently only available for shipping to the United States, Canada, and Australia.  Please contact us if you are interested in discussing distribution opportunities in your country:

I have a question that was not on this list. What do I do?

Contact us!  We would love to hear from you. Please reach out at

Where can I download full instructions for using Proov?

Full instructions on how to use Proov can be downloaded here. Additionally, each purchase of Proov will include full instructions for use.

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